DNA

Part:BBa_K2407102:Design

Designed by: Yan Zheng   Group: iGEM17_Tianjin   (2017-10-17)


control the expression of vika gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1482
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence is modified for use in yeast or other AT-rich fungi. Vika is highly specific in catalyzing vox recombination. This part expresses the vika gene which will react with vox site in the mating so as to trigger Vika-Vox system.


Source

We use overlap PCR to connect the segments(TDH3p,vika and TDH2t).

References

Karimova, M (Karimova, Madina)[ 1,2 ] ; Abi-Ghanem, J (Abi-Ghanem, Josephine)[ 3 ] ; Berger, N (Berger, Nicolas)[ 1,2 ] ; Surendranath, V (Surendranath, Vineeth)[ 4 ] ; Pisabarro, MT (Pisabarro, Maria Teresa)[ 3 ] ; Buchholz, F (Buchholz, Frank)[ 1,2 ] Vika/vox, a novel efficient and specific Cre/loxP-like site-specific recombination system 10.1093/nar/gks1037